ProductCategory: Pre-maderecombinantAAV;AAV-CAG-FLPo;AAV-CBA-FLPo;AAV5-CAG-FLPo;AAV5-CBA-FLPo
Description: FLPoriginallyidentifiedfromSaccharomycescerevisiaeisasite-specificrecombinases(SSR)fromtheλintegrasefamilyandrecognizesdistinct34bpFRTsequence.Ithasbeenwidelyusedforgeneratingconditionalknockoutmice,mediatedbyFLP/FRTsystem.InitialuseofFLPinmammaliancellsrevealedinefficientrecombinaseactivityduetoThermo-instABIlityoftheFLPprotein.Subsequentscreeningforthermo-stablemutantsresultedintheidentificationofFLPewhichwas4-foldincreaseinrecombinationefficiencyat37°C.HowevertherecombinationefficiencyofFLPeincellsremainsverylowbecauseofitsnon-mammalianorigin.
FLPoisamousecodonoptimizedFLPethatgreatlyincreasestheFRTrecombinationefficiencyforbothinvivoandinvitroinmouse. ThisAAV5-CAG-FLPopre-made rAAVinSEROtype5(withcapsidfromAAVserotype5and2xITRfromAAVserotype2)will over-expressFLPorecombinaseunderCAGpromoter(alsoknownasCBApromoter). Readytouseformat.
Titer: >1x1013 VG/mL
Size: > 30 µL(StdPack)
StorageBuffer: PBS
InfectionProtocol: 
VectorMap: 
